Anticorps Recombinant de lapin anti-HER2/ErbB2,p185-Specific

• Phare
• Validé par KD/KO

HER2/ErbB2,p185-Specific Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240802C2

N° de cat : 84046-1-PBS

Synonymes

ERBB2, 240802C2, CD340, HER 2, HER 2/neu

---

Galerie de données de validation

• 

  WB analysis using 84046-1-RR (same clone as 84046-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84046-1-RR (ERBB2,p185-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84046-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 84046-1-RR (same clone as 84046-1-PBS)

  WB result of HER2/ErbB2,p185-Specific antibody (84046-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HER2/ErbB2,p185-Specific transfected MCF-7 cells. This data was developed using the same antibody clone with 84046-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovarian cancer using 84046-1-RR (same clone as 84046-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 84046-1-RR (HER2/ErbB2,p185-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84046-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse uterus using 84046-1-RR (same clone as 84046-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse uterus tissue slide using 84046-1-RR (HER2/ErbB2,p185-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84046-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 84046-1-RR (same clone as 84046-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HER2/ErbB2,p185-Specific antibody (84046-1-RR, Clone: 240802C2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84046-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

84046-1-PBS cible HER2/ErbB2,p185-Specific dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
• Masse moléculaire calculée: 138 kDa
• Poids moléculaire observé: 185 kDa
• Numéro d’acquisition GenBank: BC167147
• Symbole du gène: HER2/ErbB2
• Identification du gène (NCBI): 2064
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand-binding domain and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signaling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas.

{{ptg:RelatedPrimaryAntibodies}}