Anticorps Monoclonal anti-ERBB2IP

• Phare
• Validé par KD/KO

ERBB2IP Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A8D4

N° de cat : 68159-1-PBS

Synonymes

LAP2, KIAA1225, ERBIN, Erbb2-interacting protein, Densin-180-like protein

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Galerie de données de validation

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  WB analysis using 68159-1-Ig (same clone as 68159-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68159-1-Ig (ERBB2IP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68159-1-PBS in a different storage buffer formulation.

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  WB analysis using 68159-1-Ig (same clone as 68159-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68159-1-Ig (ERBB2IP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68159-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68159-1-Ig (same clone as 68159-1-PBS)

  WB result of ERBB2IP antibody (68159-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERBB2IP transfected HeLa cells. This data was developed using the same antibody clone with 68159-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 68159-1-Ig (same clone as 68159-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ERBB2IP antibody (68159-1-Ig, Clone: 1A8D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68159-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51110-1

  Cytometric bead array standard curve of MP51110-1, ERBB2IP Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68159-1-PBS. Detection antibody: 68159-2-PBS. Standard:Ag18514. Range: 0.195-100 ng/mL.

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Informations sur le produit

68159-1-PBS cible ERBB2IP dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ERBB2IP Protéine recombinante Ag18514
• Nom complet: erbb2 interacting protein
• Masse moléculaire calculée: 1412 aa, 158 kDa
• Poids moléculaire observé: 150-180 kDa
• Numéro d’acquisition GenBank: BC115012
• Symbole du gène: ERBB2IP
• Identification du gène (NCBI): 55914
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ERBB2IP is a cytoplasmic protein that contains leucine-rich repeats (LRR) and PSD95/Dlg1/zo-1 (PDZ) domain (thus named a LAP protein). ERBB2IP interacts, via its single PDZ domain, specifically with ErbB2, but not with ErbB3, ErbB4, or EGFR. It is colocalized with ErbB2 at the basolateral membranes of epithelial cells. In addition, ERBB2IP also interacts with PSD‐95, Lano and with three p120‐related catenins, eBPAG1 and the β4 subunit of the α6β4 integrin, two major components of hemidesmosomes. The molecular weight of ERBB2IP was predicted 150~180 kDa by Western Blot.(PMID: 25288731,PMID: 24252174,PMID: 19289088)

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