Anticorps Polyclonal de lapin anti-ERCC1

• Phare
• Validé par KD/KO

ERCC1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 14586-1-AP

Synonymes

COFS4, ERCC1, UV20

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Galerie de données de validation

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  WB analysis using 14586-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14586-1-AP (ERCC1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 14586-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14586-1-AP (ERCC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 14586-1-AP

  IP Result of anti-ERCC1 (IP:14586-1-AP, 3ug; Detection:14586-1-AP 1:300) with MCF-7 cells lysate 1600ug.

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  IHC staining of human testis using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 14586-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7, cellules SKOV-3, cellules T-47D, tissu rénal humain
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu testiculaire humain, tissu cérébral humain, tissu cutané humain, tissu ovarien humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 13 publications below
• IHC: See 3 publications below

Informations sur le produit

14586-1-AP cible ERCC1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ERCC1 Protéine recombinante Ag6112
• Nom complet: excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC052813
• Symbole du gène: ERCC1
• Identification du gène (NCBI): 2067
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Excision Repair Cross Complementing 1 (ERCC1) is a structure-specific endonuclease that is responsible for the 5'-incision during DNA repair. It forms a complex with ERCC11, XPF and ERCC4, which are required in both recombinatorial repair and nucleotide excision repair. It has been found that ERCC1, together with RRM1, are determinants of survival after surgical treatment of early-stage, non-small-cell lung cancer. (Ref: Simon, ER. 2005). The calculated molecular weight of ERCC1 is 33 kDa, but the modified ERCC1 is about38 kDa.

Publications

Species	Application	Title
human	WB	Blood Cancer J; Severe ineffective erythropoiesis discriminates prognosis in myelodysplastic syndromes: analysis based on 776 patients from a single centre.; Authors - Huijun Huang ; KD Validated
human	WB	Cell Death Dis; PFKFB3 blockade inhibits hepatocellular carcinoma growth by impairing DNA repair through AKT.; Authors - Wen-Kai Shi
human	WB	J Cell Mol Med; Berberine attenuates XRCC1-mediated base excision repair and sensitizes breast cancer cells to the chemotherapeutic drugs.; Authors - Xingjie Gao
human	WB	Oncotarget; Functional characterization of a novel transcript of ERCC1 in chemotherapy resistance of ovarian cancer.; Authors - Jia Liu
human	WB, IHC	Oncol Res; The AKT/GSK3-Mediated Slug Expression Contributes to Oxaliplatin Resistance in Colorectal Cancer via Upregulation of ERCC1.; Authors - Wei Wei ; KD Validated
human	WB	Cancer Cell Int; Mechanistic study of lncRNA UCA1 promoting growth and cisplatin resistance in lung adenocarcinoma.; Authors - Jiali Fu