Anticorps Monoclonal anti-ERCC1

ERCC1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1H4D5

N° de cat : 66275-1-PBS

Synonymes

COFS4, ERCC1, UV20

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Galerie de données de validation

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  WB analysis of A431 using 66275-1-Ig (same clone as 66275-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66275-1-Ig (ERCC1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66275-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 66275-1-Ig (same clone as 66275-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed A431 cells using 66275-1-Ig(ERCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66275-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66275-1-PBS cible ERCC1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ERCC1 Protéine recombinante Ag6417
• Nom complet: excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC052813
• Symbole du gène: ERCC1
• Identification du gène (NCBI): 2067
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Excision Repair Cross Complementing 1 (ERCC1) is a structure-specific endonuclease that is responsible for the 5'-incision during DNA repair. It forms a complex with ERCC11, XPF and ERCC4, which are required in both recombinatorial repair and nucleotide excision repair. It has been found that ERCC1, together with RRM1, are determinants of survival after surgical treatment of early-stage, non-small-cell lung cancer. (Ref: Simon, ER. 2005)

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