Anticorps Polyclonal de lapin anti-ERCC2

ERCC2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10818-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 10818-1-AP (same clone as 10818-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10818-1-AP (ERCC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 10818-1-AP (same clone as 10818-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 10818-1-AP (ERCC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10818-1-AP (same clone as 10818-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10818-1-AP (ERCC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 10818-1-AP (same clone as 10818-1-PBS)

  IP result of anti-ERCC2 (IP:10818-1-AP, 4ug; Detection:10818-1-AP 1:1000) with HEK-293 cells lysate 1575 ug. This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 10818-1-AP (same clone as 10818-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10818-1-AP (ERCC2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 10818-1-AP (same clone as 10818-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10818-1-AP (ERCC2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 10818-1-AP (same clone as 10818-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10818-1-AP (ERCC2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 10818-1-AP (same clone as 10818-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10818-1-AP (ERCC2 antibody) at dilution of 1:25 (under 10x lens). This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 10818-1-AP (same clone as 10818-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10818-1-AP (ERCC2 antibody) at dilution of 1:25 (under 40x lens). This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10818-1-PBS cible ERCC2 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ERCC2 Protéine recombinante Ag1038
• Nom complet: excision repair cross-complementing rodent repair deficiency, complementation group 2
• Masse moléculaire calculée: 87 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC008346
• Symbole du gène: ERCC2
• Identification du gène (NCBI): 2068
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Excision-repair complementing defective in Chinese hamster 2(ERCC2), belongs to the nucleotide excision repair pathway, is a component of the TFIIH transcription complex and required for the association of the CDK-activating kinase(CAK) subcomplex with TFIIH. Since TFIIH is involved in both DNA repair and cell cycle progression, ERCC2 is implicated to play an integral role in the nucleotide excision repair pathway. Also it involves in the regulation of vitamin-D receptor activity. As a part of the mitotic spindle-associated MMXD complex, ERCC2 has a role in chromosome segregation.

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