Anticorps Polyclonal de lapin anti-ERCC3

• Phare
• Validé par KD/KO

ERCC3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10580-1-PBS

Synonymes

EC:5.6.2.4, DNA repair protein complementing XP-B cells, DNA excision repair protein ERCC-3, DNA 3'-5' helicase/translocase XPB, BTF2 p89

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Galerie de données de validation

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  WB analysis of COLO 320 using 10580-1-AP (same clone as 10580-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10580-1-AP (same clone as 10580-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10580-1-AP (same clone as 10580-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10580-1-AP (same clone as 10580-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 10580-1-AP (same clone as 10580-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse colon using 10580-1-AP (same clone as 10580-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-1080 using 10580-1-AP (same clone as 10580-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10580-1-AP (same clone as 10580-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10580-1-AP (same clone as 10580-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

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  IP experiment of A431 using 10580-1-AP (same clone as 10580-1-PBS)

  IP result of anti-ERCC3 (IP:10580-1-AP, 4ug; Detection:10580-1-AP 1:500) with A431 cells lysate 1200ug. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 10580-1-AP (same clone as 10580-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10580-1-AP (ERCC3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10580-1-PBS cible ERCC3 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ERCC3 Protéine recombinante Ag0876
• Nom complet: excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing)
• Masse moléculaire calculée: 89 kDa
• Poids moléculaire observé: 89 kDa
• Numéro d’acquisition GenBank: BC008820
• Symbole du gène: ERCC3
• Identification du gène (NCBI): 2071
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Excision repair cross-complementing 3/xeroderma pigmentosum B (ERCC3/XPB) DNA helicase, is an essential ATP-dependent DNA helicase that functions as an integral component of the transcription factor IIH complex. ERCC3 is involved in initiation of basal transcription and global genome repair as well as in transcription-coupled repair (TCR) [PMID:19942596]. At the cellular level, a defect in ERCC3 results in high UV sensitivity, reduced post-UV RNA synthesis recovery and decreased DNA repair due to defective NER [PMID:16947863].

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