Anticorps Polyclonal de lapin anti-XPG

• Phare
• Validé par KD/KO

XPG Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(14)

N° de cat : 11331-1-AP

Synonymes

ERCC5, COFS3, DNA excision repair protein ERCC-5, DNA repair protein complementing XP-G cells, EC:3.1.-.-

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Galerie de données de validation

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  WB analysis using 11331-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11331-1-AP (XPG antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11331-1-AP

  IP result of anti-XPG (IP:11331-1-AP, 4ug; Detection:11331-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IF Staining of HeLa using 11331-1-AP

  Immunofluorescent analysis of HeLa cells using 11331-1-AP (XPG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Daudi, cellules HepG2
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IF: See 1 publications below

Informations sur le produit

11331-1-AP cible XPG dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: XPG Protéine recombinante Ag1874
• Nom complet: excision repair cross-complementing rodent repair deficiency, complementation group 5
• Masse moléculaire calculée: 1186 aa, 133 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: BC031522
• Symbole du gène: ERCC5
• Identification du gène (NCBI): 2073
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The human genes correcting DNA repair defects are termed excision-repair cross-complementing or ERCC genes. The ERCC5 gene corrects the excision repair deficiency of Chinese hamster ovary cell line UV135 of complementation group 5. The human ERCC5 gene product is a structure-specific endonuclease required for making the 3-prime incision during DNA nucleotide excision-repair (NER). It also plays an important role in regulating DNA excision repair, removal of bulky lesions caused by environmental chemicals or UV light [PMID:22815677]. The calculated molecular weight of ERCC5 is 133 kDa, but the modified ERCC5 protein is about 200 kDa.

Publications

Species	Application	Title
human	WB	Cell; Human Rad52 Promotes XPG-Mediated R-loop Processing to Initiate Transcription-Associated Homologous Recombination Repair.; Authors - Takaaki Yasuhara ; KD Validated
human		Cancer Cell; Systematic Functional Annotation of Somatic Mutations in Cancer.; Authors - Patrick Kwok-Shing Ng
		Mol Carcinog; Associations between Expression Levels of Nucleotide Excision Repair Proteins in Lymphoblastoid Cells and Risk of Squamous Cell Carcinoma of the Head and Neck.; Authors - Peng Han
human	WB	Oncol Rep; Characterization of the nucleotide excision repair pathway and evaluation of compounds for overcoming the cisplatin resistance of non‑small cell lung cancer cell lines.; Authors - Toshihiro Suzuki ; KD Validated
human	WB	J Cancer; PMID: 31772670; Authors - Li-Ming Tan
human	WB	Biochim Biophys Acta; Stimulation of ribosomal RNA gene promoter by transcription factor Sp1 involves active DNA demethylation by Gadd45-NER pathway.; Authors - Pallavi Rajput

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