Anticorps Monoclonal anti-ERCC5

ERCC5 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1G2C9

N° de cat : 67055-1-Ig

Synonymes

XPG, 1G2C9, COFS3, DNA excision repair protein ERCC-5, DNA repair protein complementing XP-G cells

---

Galerie de données de validation

• 

  WB analysis using 67055-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67055-1-Ig (ERCC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 67055-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 67055-1-Ig (ERCC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 67055-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 67055-1-Ig (ERCC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 67055-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67055-1-Ig (ERCC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules HT-29, cellules COLO 320, cellules Daudi, cellules HeLa, cellules HepG2, cellules Jurkat, cellules Ramos

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67055-1-Ig cible ERCC5 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ERCC5 Protéine recombinante Ag28561
• Nom complet: excision repair cross-complementing rodent repair deficiency, complementation group 5
• Masse moléculaire calculée: 1186 aa, 133 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: BC031522
• Symbole du gène: ERCC5
• Identification du gène (NCBI): 2073
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The human genes correcting DNA repair defects are termed excision-repair cross-complementing or ERCC genes. The ERCC5 gene corrects the excision repair deficiency of Chinese hamster ovary cell line UV135 of complementation group 5. The human ERCC5 gene product is a structure-specific endonuclease required for making the 3-prime incision during DNA nucleotide excision-repair (NER). It also plays an important role in regulating DNA excision repair, removal of bulky lesions caused by environmental chemicals or UV light [PMID:22815677]. The calculated molecular weight of ERCC5 is 133 kDa, but the modified ERCC5 protein is about 200 kDa.

Publications

Species	Application	Title
human	WB	Front Pharmacol; Triptolide enhances carboplatin-induced apoptosis by inhibiting nucleotide excision repair (NER) activity in melanoma; Authors - Geng Wang

{{ptg:RelatedPrimaryAntibodies}}