Anticorps Monoclonal anti-ERCC5

ERCC5 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G2C9

N° de cat : 67055-1-PBS

Synonymes

XPG, 1G2C9, COFS3, DNA excision repair protein ERCC-5, DNA repair protein complementing XP-G cells

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Galerie de données de validation

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  WB analysis using 67055-1-Ig (same clone as 67055-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67055-1-Ig (ERCC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67055-1-PBS in a different storage buffer formulation.

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  WB analysis of Daudi using 67055-1-Ig (same clone as 67055-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 67055-1-Ig (ERCC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67055-1-PBS in a different storage buffer formulation.

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  WB analysis of Ramos using 67055-1-Ig (same clone as 67055-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 67055-1-Ig (ERCC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67055-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 67055-1-Ig (same clone as 67055-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67055-1-Ig (ERCC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67055-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67055-1-PBS cible ERCC5 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ERCC5 Protéine recombinante Ag28561
• Nom complet: excision repair cross-complementing rodent repair deficiency, complementation group 5
• Masse moléculaire calculée: 1186 aa, 133 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: BC031522
• Symbole du gène: ERCC5
• Identification du gène (NCBI): 2073
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The human genes correcting DNA repair defects are termed excision-repair cross-complementing or ERCC genes. The ERCC5 gene corrects the excision repair deficiency of Chinese hamster ovary cell line UV135 of complementation group 5. The human ERCC5 gene product is a structure-specific endonuclease required for making the 3-prime incision during DNA nucleotide excision-repair (NER). It also plays an important role in regulating DNA excision repair, removal of bulky lesions caused by environmental chemicals or UV light [PMID:22815677]. The calculated molecular weight of ERCC5 is 133 kDa, but the modified ERCC5 protein is about 200 kDa.

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