Anticorps Recombinant de lapin anti-ERCC6L

ERCC6L Recombinant Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251669A8

N° de cat : 86688-1-PBS

Synonymes

ATP-dependent helicase ERCC6-like, DNA excision repair protein ERCC-6-like, EC:3.6.4.12, PICH, PLK1-interacting checkpoint helicase

---

Galerie de données de validation

• 

  WB analysis using 86688-1-RR (same clone as 86688-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86688-1-RR (ERCC6L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86688-1-PBS in a different storage buffer formulation.

• 

  IP experiment of K-562 using 86688-1-RR (same clone as 86688-1-PBS)

  IP result of anti-ERCC6L (IP:86688-1-RR, 4ug; Detection:86688-1-RR 1:2000) with K-562 cells lysate 1640 ug. This data was developed using the same antibody clone with 86688-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 86688-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86688-1-RR against Human ERCC6L were performed. The affinity constant is 0.79 nM.

---

Informations sur le produit

86688-1-PBS cible ERCC6L dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ERCC6L Protéine recombinante Ag8223
• Nom complet: excision repair cross-complementing rodent repair deficiency, complementation group 6-like
• Masse moléculaire calculée: 419 aa, 46 kDa, 141 kDa
• Poids moléculaire observé: 180 kDa
• Numéro d’acquisition GenBank: BC008808
• Symbole du gène: ERCC6L
• Identification du gène (NCBI): 54821
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ERCC6L, also named as Tumor antigen BJ-HCC-15 or PICH, is a 1250 amino acid protein, which contains one helicase ATP-binding domain, one helicase C-terminal domain and two TPR repeats. ERCC6L localizes to kinetochores, inner centromeres and belongs to the SNF2/RAD54 helicase family. ERCC6L as a DNA helicase that acts as an essential component of the spindle assembly checkpoint. The calculated molecular weight of ERCC6L is 140 kDa, but the phosphorylated modification of molecular weight of ERCC6L is about 180kDa.

{{ptg:RelatedPrimaryAntibodies}}