Anticorps Polyclonal de lapin anti-ERK1/2

ERK1/2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 51068-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 51068-1-AP (same clone as 51068-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 51068-1-AP (ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

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  WB analysis using 51068-1-AP (same clone as 51068-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 51068-1-AP (ERK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 51068-1-AP (same clone as 51068-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51068-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 51068-1-AP (same clone as 51068-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51068-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 51068-1-AP (same clone as 51068-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51068-1-AP (ERK1/2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 51068-1-AP (same clone as 51068-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51068-1-AP (ERK1/2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 51068-1-AP (same clone as 51068-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ERK1/2 antibody (51068-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 51068-1-AP (same clone as 51068-1-PBS)

  Immunofluorescent analysis of HeLa cells using 51068-1-AP (ERK1/2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

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Informations sur le produit

51068-1-PBS cible ERK1/2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mitogen-activated protein kinase 1
• Masse moléculaire calculée: 360 aa, 41 kDa
• Poids moléculaire observé: 38-44 kDa
• Numéro d’acquisition GenBank: BC017832
• Symbole du gène: ERK2
• Identification du gène (NCBI): 5594
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

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