Anticorps Monoclonal anti-ERK1/2

ERK1/2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4G8A11

N° de cat : 66192-1-PBS

Synonymes

ERK, ERK1, ERK2, 4G8A11, EC:2.7.11.24

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Galerie de données de validation

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  WB analysis using 66192-1-Ig (same clone as 66192-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66192-1-Ig (ERK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66192-1-Ig (same clone as 66192-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66192-1-Ig (same clone as 66192-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66192-1-Ig (same clone as 66192-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66192-1-Ig (same clone as 66192-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66192-1-Ig (same clone as 66192-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66192-1-Ig (same clone as 66192-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66192-1-Ig (same clone as 66192-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66192-1-Ig (same clone as 66192-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66192-1-Ig (same clone as 66192-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66192-1-Ig (same clone as 66192-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ERK1/2 antibody (66192-1-Ig, Clone: 4G8A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66192-1-PBS cible ERK1/2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ERK1/2 Protéine recombinante Ag20350
• Nom complet: mitogen-activated protein kinase 3
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 38-44 kDa
• Numéro d’acquisition GenBank: BC013992
• Symbole du gène: ERK1
• Identification du gène (NCBI): 5595
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

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