Anticorps Recombinant de lapin anti-ERK1/2

ERK1/2 Recombinant Antibody for WB, IHC, IHC-Autostainer, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IHC-Autostainer, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

4P8

N° de cat : 83533-1-PBS

Synonymes

ERK, ERK1, ERK2, 4P8, EC:2.7.11.24

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Galerie de données de validation

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  WB analysis using 83533-1-RR (same clone as 83533-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83533-1-RR (ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83533-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 83533-1-RR (same clone as 83533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 83533-1-RR (ERK1/2 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Staining was performed on the Epredia 480S Autostainer. This data was developed using the same antibody clone with 83533-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 83533-1-RR (same clone as 83533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 83533-1-RR (ERK1/2 antibody) at dilution of 1:100 (under 40x lens).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Staining was performed on the Epredia 480S Autostainer.​ This data was developed using the same antibody clone with 83533-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 83533-1-RR (same clone as 83533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 83533-1-RR (ERK1/2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83533-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 83533-1-RR (same clone as 83533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 83533-1-RR (ERK1/2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83533-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 83533-1-RR (same clone as 83533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83533-1-RR (ERK1/2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83533-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 83533-1-RR (same clone as 83533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83533-1-RR (ERK1/2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83533-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83533-1-RR (same clone as 83533-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ERK1/2 antibody (83533-1-RR, Clone: 4P8 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83533-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83533-1-RR (same clone as 83533-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ERK1/2 Recombinant antibody (83533-1-RR, Clone:4P8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83533-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83533-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83533-1-RR against Human ERK1/2 were performed. The affinity constant is 17.8 nM.

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Informations sur le produit

83533-1-PBS cible ERK1/2 dans les applications de WB, IHC, IHC-Autostainer, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ERK1/2 Protéine recombinante Ag20350
• Nom complet: mitogen-activated protein kinase 3
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 38-44 kDa
• Numéro d’acquisition GenBank: BC013992
• Symbole du gène: ERK1
• Identification du gène (NCBI): 5595
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

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