Anticorps Polyclonal de lapin anti-ERK1/2

ERK1/2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

26

N° de cat : 51068-1-AP

Synonymes

ERK, ERK 2, ERK1/2, ERK2, ERT1, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAPK 1, MAPK 2, MAPK1, MAPK2, p40, p41, p41mapk, p42 MAPK, P42MAPK, PRKM1, PRKM2

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Galerie de données de validation

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  WB analysis using 51068-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51068-1-AP (ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 51068-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51068-1-AP (ERK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 51068-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51068-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 51068-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51068-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 51068-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51068-1-AP (ERK1/2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervical cancer using 51068-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51068-1-AP (ERK1/2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 51068-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ERK1/2 antibody (51068-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 51068-1-AP

  Immunofluorescent analysis of HeLa cells using 51068-1-AP (ERK1/2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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  FC experiment of HeLa using 51068-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human ERK1/2 (51068-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules Jurkat, cellules NIH/3T3, tissu cérébral de souris, tissu cutané de rat
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 23 publications below
• IHC: See 3 publications below

Informations sur le produit

51068-1-AP cible ERK1/2 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mitogen-activated protein kinase 1
• Masse moléculaire calculée: 360 aa, 41 kDa
• Poids moléculaire observé: 38-44 kDa
• Numéro d’acquisition GenBank: BC017832
• Symbole du gène: MAPK1
• Identification du gène (NCBI): 5594
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

Publications

Species	Application	Title
human	WB	Int J Biol Sci; FLOT1 promotes gastric cancer progression and metastasis through BCAR1/ERK signaling; Authors - Ran Wang
human	WB	Cell Death Dis; USP4 promotes the proliferation, migration, and invasion of esophageal squamous cell carcinoma by targeting TAK1; Authors - Hongdian Zhang
mouse,human	WB	Cell Rep; Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism; Authors - Hao Qin
human	WB	Pharmacol Res; The PI3Kα inhibitor DFX24 suppresses tumor growth and metastasis in non-small cell lung cancer via ERK inhibition and EPHB6 reactivation.; Authors - Yan-Hua Fan
mouse	WB	Cell Cycle; Urokinase-type plasminogen activator receptor signaling is critical in nasopharyngeal carcinoma cell growth and metastasis.; Authors - Ying-Na Bao
mouse	WB	Biochem Pharmacol; Tangeretin prevents cognitive deficit in delirium through activating RORα/γ-E4BP4 axis in mice; Authors - Min Chen