Anticorps Polyclonal de lapin anti-ERMN

ERMN Polyclonal Antibody for WB, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31432-1-PBS

Synonymes

KIAA1189, Juxtanodin, JN, Ermin

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Galerie de données de validation

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  WB analysis using 31432-1-AP (same clone as 31432-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31432-1-AP (ERMN antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31432-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 31432-1-AP (same clone as 31432-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using ERMN antibody (31432-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31432-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 31432-1-AP (same clone as 31432-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using ERMN antibody (31432-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31432-1-PBS in a different storage buffer formulation.

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Informations sur le produit

31432-1-PBS cible ERMN dans les applications de WB, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ERMN Protéine recombinante Ag35648
• Nom complet: ermin, ERM-like protein
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC026345
• Symbole du gène: ERMN
• Identification du gène (NCBI): 57471
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ERMN is associated with cytoskeletal rearrangements and the stability of myelin sheath. A study has shown that ERMN is expressed specifically in oligodendrocytes in the central nervous system (CNS), and is involved in the formation of myelin (PMID: 20934411). The predicted MW of ERMN is 33 KDa, while western blot analyses detected a 45-50 kDa single band protein as a result of phosphorylation modification.

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