Anticorps Monoclonal anti-ERO1L

• Phare
• Validé par KD/KO

ERO1L Monoclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1G12E11

N° de cat : 67416-1-Ig

Synonymes

ERO1 alpha, ERO1 L, ERO1 L alpha, ERO1 like (S. cerevisiae), ERO1 like protein alpha, ERO1L, Oxidoreductin 1 L alpha

---

Galerie de données de validation

• 

  WB analysis of HepG2 using 67416-1-Ig

  WB result of ERO1L antibody (67416-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERO1L transfected HepG2 cells.

• 

  WB analysis using 67416-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67416-1-Ig (ERO1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 67416-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67416-1-Ig (ERO1L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 67416-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67416-1-Ig (ERO1L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 67416-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67416-1-Ig (ERO1L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 67416-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67416-1-Ig (ERO1L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 67416-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using ERO1L antibody (67416-1-Ig, Clone: 1G12E11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human stomach cancer using 67416-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human stomach cancer tissue using ERO1L antibody (67416-1-Ig, Clone: 1G12E11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules 4T1, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293, tissu de cancer de l'estomac humain

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67416-1-Ig cible ERO1L dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ERO1L Protéine recombinante Ag29910
• Nom complet: ERO1-like (S. cerevisiae)
• Masse moléculaire calculée: 468 aa, 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC008674
• Symbole du gène: ERO1L
• Identification du gène (NCBI): 30001
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ERO1L, also named as ERO1-alpha, is an essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. It acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. It does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Glutathione may be required to regulate its activity in the endoplasmic reticulum. It may be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. It is required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. ERO1L has a calculated molecular weight of 54 kDa and can be detected as 60kDa.

Publications

Species	Application	Title
mouse	WB	Theranostics; A novel NEDD4L-TXNIP-CHOP axis in the pathogenesis of nonalcoholic steatohepatitis; Authors - Qian Guo