Anticorps Polyclonal de lapin anti-ERO1LB

ERO1LB Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18312-1-PBS

Synonymes

ERO1-L-beta, Ero1beta, ERO1B, Endoplasmic reticulum oxidoreductin-1-like protein B, Endoplasmic reticulum oxidoreductase beta

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Galerie de données de validation

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  WB analysis of human brain using 18312-1-AP (same clone as 18312-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 18312-1-AP (ERO1LB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18312-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 18312-1-AP (same clone as 18312-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 18312-1-AP (ERO1LB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18312-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 18312-1-AP (same clone as 18312-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18312-1-AP (ERO1LB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18312-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 18312-1-AP (same clone as 18312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18312-1-AP (ERO1LB Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18312-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 18312-1-AP (same clone as 18312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18312-1-AP (ERO1LB Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18312-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18312-1-PBS cible ERO1LB dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ERO1-like beta (S. cerevisiae)
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC032823
• Symbole du gène: ERO1LB
• Identification du gène (NCBI): 56605
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ERO1LB is an essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. It acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. It does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Glutathione may be required to regulate its activity in the endoplasmic reticulum. It may be responsible for a significant proportion of reactive oxygen species (ROS) in the being a source of oxidative stress. It is required for the folding of cell, thereby being a source of oxidative stress. This antibody has no cross reaction to ERO1L.

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