Anticorps Monoclonal anti-ERP29

ERP29 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1H8B2

N° de cat : 67675-1-PBS

Synonymes

C12orf8, ERp28, ERP29, ERp31, PDI DB, PDIA9

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Galerie de données de validation

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  WB analysis using 67675-1-Ig (same clone as 67675-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67675-1-Ig (ERP29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67675-1-Ig (same clone as 67675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67675-1-Ig (ERP29 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67675-1-Ig (same clone as 67675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67675-1-Ig (ERP29 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67675-1-Ig (same clone as 67675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67675-1-Ig (ERP29 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 67675-1-Ig (same clone as 67675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67675-1-Ig (ERP29 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

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  IF Staining of human kidney using 67675-1-Ig (same clone as 67675-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ERP29 antibody (67675-1-Ig, Clone: 1H8B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human kidney using 67675-1-Ig (same clone as 67675-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ERP29 antibody (67675-1-Ig, Clone: 1H8B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67675-1-PBS cible ERP29 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ERP29 Protéine recombinante Ag30262
• Nom complet: endoplasmic reticulum protein 29
• Masse moléculaire calculée: 261 aa, 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC101495
• Symbole du gène: ERP29
• Identification du gène (NCBI): 10961
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Endoplasmic reticulum resident protein 29 (ERP29) is a 29-kDa endoplasmic reticulum (ER) luminal protein. Structurally ERp29 is related to protein disulfide isomerases (PDI). However, it lacks the thioredoxin motif, suggesting that this protein does not function as a disulfide isomerase (PMID: 15500441). ERP29 plays an important role in the processing of secretory proteins within the (ER), possibly by participating in the folding of proteins in the ER. The secretion of thyroglobulin (TG) was found to be strongly regulated by ERP29 (PMID: 16380091).

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