Anticorps Monoclonal anti-ERp57/ERp60

ERp57/ERp60 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3G4G7

N° de cat : 66423-1-PBS

Synonymes

ERp57, PDIA3, 3G4G7, 58 kDa glucose-regulated protein, Disulfide isomerase ER-60

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Galerie de données de validation

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  WB analysis using 66423-1-Ig (same clone as 66423-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66423-1-Ig (same clone as 66423-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66423-1-Ig (same clone as 66423-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66423-1-Ig (same clone as 66423-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66423-1-Ig (same clone as 66423-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66423-1-Ig (same clone as 66423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66423-1-Ig (same clone as 66423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66423-1-Ig (same clone as 66423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66423-1-Ig (same clone as 66423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66423-1-Ig (same clone as 66423-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ERp57/ERp60 antibody (66423-1-Ig, Clone: 3G4G7 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66423-1-PBS cible ERp57/ERp60 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ERp57/ERp60 Protéine recombinante Ag8741
• Nom complet: protein disulfide isomerase family A, member 3
• Masse moléculaire calculée: 505 aa, 57 kDa
• Poids moléculaire observé: 57 kDa, 60 kDa
• Numéro d’acquisition GenBank: BC014433
• Symbole du gène: PDIA3
• Identification du gène (NCBI): 2923
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

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