Anticorps Monoclonal anti-ERp72

ERp72 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D5F3

N° de cat : 66365-1-PBS

Synonymes

Cell and organelle markers, endoplasmic reticulum marker, ER marker, ER protein 70, ER protein 72, ERp 72, ERP70, ERp72, PDIA4, Protein disulfide isomerase A4

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Galerie de données de validation

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  WB analysis using 66365-1-Ig (same clone as 66365-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66365-1-Ig (ERp72 antibody) at dilution of 1:4900 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66365-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66365-1-Ig (same clone as 66365-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66365-1-Ig (ERp72 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66365-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66365-1-Ig (same clone as 66365-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66365-1-Ig (ERp72 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66365-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66365-1-PBS cible ERp72 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ERp72 Protéine recombinante Ag6873
• Nom complet: protein disulfide isomerase family A, member 4
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 72 kDa
• Numéro d’acquisition GenBank: BC000425
• Symbole du gène: ERp72
• Identification du gène (NCBI): 9601
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PDIA4 (Protein disulfide-isomerase A4) is also named ERP70, ERP72, and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full-length protein has a signal peptide with 20 amino acids.

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