Anticorps Polyclonal de lapin anti-ESCO2

ESCO2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 23525-1-AP

Synonymes

N acetyltransferase ESCO2, hEFO2, Establishment factor-like protein 2, EFO2p, EFO2

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Galerie de données de validation

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  WB analysis using 23525-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23525-1-AP (ESCO2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 23525-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23525-1-AP (ESCO2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 23525-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 23525-1-AP (ESCO2 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, testicule de rat, testicule de souris
• Résultats positifs en IHC: tissu de cancer de la peau humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

23525-1-AP cible ESCO2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ESCO2 Protéine recombinante Ag18893
• Nom complet: establishment of cohesion 1 homolog 2 (S. cerevisiae)
• Masse moléculaire calculée: 601 aa, 68 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC146562
• Symbole du gène: ESCO2
• Identification du gène (NCBI): 157570
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ESCO2 is also known as Establishment of Sister Chromatid Cohesion N-Acetyltransferase 2, EFO2. This gene encodes a member of the family of acetyltransferases involved in the establishment of sister chromatid cohesion during S phase and postreplicative sister chromatid cohesion induced by double-strand breaks (PubMed: 15821733). Mutations in the ESCO2 gene are associated with a rare genetic disorder called Roberts syndrome or SC phocomelia syndrome (PubMed: 16380922). The loss of function or impaired activity of the ESCO2 protein due to mutations disrupts the proper cohesion of sister chromatids, leading to chromosomal abnormalities and the characteristic features of Roberts syndrome. ESCO2 can be detected as about 65-70 kDa.

Publications

Species	Application	Title
human	WB	Kaohsiung J Med Sci; Long noncoding RNA ZFPM2-AS1 regulates renal cell carcinoma progression via miR-130a-3p/ESCO2.; Authors - Gang Zhang
human	WB	BMC Cancer; ESCO2's oncogenic role in human tumors: a pan-cancer analysis and experimental validation; Authors - Yue Huang
human	WB	Proc Natl Acad Sci U S A; Lactylation of RNA m6A demethylase ALKBH5 promotes innate immune response to DNA herpesviruses and mpox virus; Authors - Wan Li
bovine	IF	J Dairy Sci; Identification of Candidate Genes Related to Hybrid Sterility by Genomic Structural Variation and Transcriptome Analyses in Cattle-yak; Authors - Rui-Dong Wan
mouse	WB	Cell Prolif; METTL16 and YTHDC1 Regulate Spermatogonial Differentiation via m6A; Authors - Xueying Gu

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