Anticorps Monoclonal anti-ESD

ESD Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

2F5E1

N° de cat : 67069-2-PBS

Synonymes

Methylumbelliferyl-acetate deacetylase, FGH, Esterase D, EC:3.1.2.12, EC:3.1.1.56

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Galerie de données de validation

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  WB analysis using 67069-2-Ig (same clone as 67069-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67069-2-Ig (ESD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67069-2-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 67069-2-Ig (same clone as 67069-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ESD antibody (67069-2-Ig, Clone: 2F5E1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67069-2-PBS in a different storage buffer formulation.

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Informations sur le produit

67069-2-PBS cible ESD dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ESD Protéine recombinante Ag7487
• Nom complet: esterase D/formylglutathione hydrolase
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 30-34 kDa
• Numéro d’acquisition GenBank: BC001169
• Symbole du gène: ESD
• Identification du gène (NCBI): 2098
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Esterase D (ESD) is a non-specific esterase widely distributed in various organisms and is also named S-Formylglutathione Hydrolase (SFGH). ESD is a member of the carboxylesterase family and has both carboxylesterase and thioesterase activities. ESD plays an important role in the process of glutathione-dependent detoxification, regulating cholesterol efflux and virus infection in humans, and is closely related to the development of tumors. ESD as a Genetic Marker for Retinoblastoma (PMID: 32247735, PMID: 34875997, PMID: 35627173). The calculated molecular weight of ESD is 31 kDa.

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