Anticorps Polyclonal de lapin anti-ESM1

ESM1 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12355-1-PBS

Synonymes

endocan, endothelial cell molecule 1, Endothelial cell specific molecule 1, Endothelial cell-specific molecule 1, ESM-1

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Galerie de données de validation

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  WB analysis of HUVEC using 12355-1-AP (same clone as 12355-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 12355-1-AP (ESM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12355-1-PBS in a different storage buffer formulation.

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  IP experiment of HUVEC using 12355-1-AP (same clone as 12355-1-PBS)

  IP result of anti-ESM1 (IP:12355-1-AP, 4ug; Detection:12355-1-AP 1:300) with HUVEC cells lysate 640 ug. This data was developed using the same antibody clone with 12355-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12355-1-PBS cible ESM1 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ESM1 Protéine recombinante Ag3019
• Nom complet: endothelial cell-specific molecule 1
• Masse moléculaire calculée: 184 aa, 20 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC011989
• Symbole du gène: ESM1
• Identification du gène (NCBI): 11082
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ESM1 is a secreted protein mainly expressed in the endothelial cells. The deduced 184-amino acid protein is cysteine rich with an N-terminal hydrophobic signal sequence. Expression of ESM1 was tested by Northern blot analysis and RT-PCR and was found to be constitutive in a HUVEC cell line, but not in any other cell line tested. Constitutive ESM1 expression in human tissue was restricted to lung.

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