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Anticorps Polyclonal de lapin anti-ESPL1

ESPL1 Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 22261-1-PBS

Synonymes

Caspase like protein ESPL1, ESP1, KIAA0165, SEPARASE, SEPARIN

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using ESPL1 Polyclonal antibody (22261-1-AP)

    WB analysis using 22261-1-AP (same clone as 22261-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22261-1-AP (ESPL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22261-1-PBS in a different storage buffer formulation.

Informations sur le produit

22261-1-PBS cible ESPL1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet extra spindle pole bodies homolog 1 (S. cerevisiae)
Masse moléculaire calculée 233 kDa
Poids moléculaire observé170 kDa
Numéro d’acquisition GenBankNM_012291
Symbole du gène ESPL1
Identification du gène (NCBI) 9700
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ESPL1 (Extra spindle poles-like 1 protein), also known as Separin, Separase, a cysteine endopeptidase, plays a vital role in the stable binding between sister chromatids before anaphase and their timely separation during anaphase, which is the key to chromosome inheritance (PMID: 34512713). ESPL1 expression is elevated and predicts poor outcomes in patients with various types of cancer, including breast cancer, glioma and endometrial cancer (PMID: 36465268). Full-length ESPL1 decreased after 30 min resulting in a ~ 170 kDa N-terminal (ESPL1N−term) and a ~ 60 kDa C-terminal polypeptide (ESPL1CC−term), reflecting ESPL1 activation and auto-cleavage (PMID: 30429481)

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