Anticorps Polyclonal de lapin anti-ESR2

• Phare
• Validé par KD/KO

ESR2 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

56

Avis (2)

N° de cat : 14007-1-AP

Synonymes

ER BETA, Erb, ESR BETA, ESR2, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, Estrogen Receptor β, NR3A2

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Galerie de données de validation

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  WB analysis using 14007-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14007-1-AP (ESR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14007-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 14007-1-AP (ESR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 14007-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14007-1-AP (ESR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14007-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14007-1-AP (ESR2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse ovary using 14007-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14007-1-AP (ESR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 14007-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14007-1-AP (ESR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules NIH/3T3, cellules PC-3, cellules SH-SY5Y, cellules SKOV-3, tissu cérébral de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu ovarien de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 40 publications below
• IHC: See 23 publications below
• IF: See 9 publications below

Informations sur le produit

14007-1-AP cible ESR2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ESR2 Protéine recombinante Ag5103
• Nom complet: estrogen receptor 2 (ER beta)
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: BC024181
• Symbole du gène: ESR2
• Identification du gène (NCBI): 2100
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. ESR2 exists various isoforms and range of calculated molecular weight of isoforms is 50-60 kDa. The experiment detected two bands for ESR2 in isolated human germ cells, one at 60 kDa and a weaker one at 50 kDa(PMID:14766008).

Publications

Species	Application	Title
mouse	WB	J Biomed Sci; Dioscin promotes osteoblastic proliferation and differentiation via Lrp5 and ER pathway in mouse and human osteoblast-like cell lines.; Authors - Chunfang Zhang
human	IF	ACS Sens; Estrogen Receptor β-Targeted Near-Infrared Inherently Fluorescent Probe: A Potent Tool for Estrogen Receptor β Research.; Authors - Qiuyu Meng
human	WB,IHC	Int J Cancer; ERβ promoted invadopodia formation-mediated non-small cell lung cancer metastasis via the ICAM1/p-Src/p-Cortactin signaling pathway; Authors - Yangwei Wang ; KD Validated
mouse	IHC	Int J Cancer; Estrogen and insulin-like growth factor synergistically promote the development of lung adenocarcinoma in mice.; Authors - Tang Hexiao H
human	WB	Cell Death Dis; ERβ promotes Aβ degradation via the modulation of autophagy.; Authors - Yong Wei
human	WB,IHC	Fertil Steril; Elevated maternal androgen is associated with dysfunctional placenta and lipid disorder in newborns of mothers with polycystic ovary syndrome.; Authors - Miao Sun

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Anna (Verified Customer) (10-04-2022)

No specific reaction - positive reaction should be visible only in nucleus but in our staining is present also in the cytoplasm. Moreover, we used the material from knockout mice without ESR2 and we observed response in this slices to this receptor. I have a problem with attaching a photo file. Please check the file below. https://drive.google.com/file/d/15d3t6thx7rcWyN-_DkkKCtpAMyi8jxHO/view

• Applications: Immunofluorescence
• Primary Antibody Dilution: 100, 200
• Cell Tissue Type: brain frozen sections - mouse

FH

Kenneth (Verified Customer) (01-08-2020)

N/A

• Applications: Western Blot,

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