Anticorps Monoclonal anti-ESR2

• Phare
• Validé par KD/KO

ESR2 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5D2C9

N° de cat : 60197-1-PBS

Synonymes

ER BETA, Erb, ERβ, ESR BETA, ESR2, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, NR3A2

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Galerie de données de validation

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  WB analysis of MCF-7 using 60197-1-Ig (same clone as 60197-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60197-1-Ig (ESR2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60197-1-PBS in a different storage buffer formulation.

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  WB analysis of SW 1990 using 60197-1-Ig (same clone as 60197-1-PBS)

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 60197-1-Ig (ESR2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60197-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 60197-1-Ig (same clone as 60197-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 60197-1-Ig (ESR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60197-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60197-1-PBS cible ESR2 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ESR2 Protéine recombinante Ag5103
• Nom complet: estrogen receptor 2 (ER beta)
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: BC024181
• Symbole du gène: ESR2
• Identification du gène (NCBI): 2100
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. ESR2 exists various isoform and range of calculated molecular weight of isoforms is 50-60 kDa. The experiment detected two bands for ESR2 in isolated human germ cells, one at 60 kDa and a weaker one at 50 kDa (PMID: 14766008).

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