Anticorps Recombinant de lapin anti-ESR2

ESR2 Recombinant Antibody for IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240412F2

N° de cat : 83447-3-PBS

Synonymes

240412F2, ER BETA, Erb, ER-beta, ERβ

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Galerie de données de validation

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  IHC staining of mouse testis using 83447-3-RR (same clone as 83447-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83447-3-RR (ESR2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83447-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 83447-3-RR (same clone as 83447-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 83447-3-RR (ESR2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83447-3-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83447-3-RR (same clone as 83447-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ESR2 antibody (83447-3-RR, Clone: 240412F2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83447-3-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 83447-3-RR (same clone as 83447-3-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Esr2 Recombinant Antibody (83447-3-RR, Clone:240412F2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83447-3-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83447-3-RR (same clone as 83447-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Esr2 Recombinant Antibody (83447-3-RR, Clone:240412F2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83447-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83447-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 83447-3-PBS against Human ESR2 was performed. The affinity constant is 16.6 pM.

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  Affinity and Kinetic Characterization of 83447-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83447-3-RR against Human ESR2 were performed. The affinity constant is 0.22 nM.

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Informations sur le produit

83447-3-PBS cible ESR2 dans les applications de IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ESR2 Protéine recombinante Ag5103
• Nom complet: estrogen receptor 2 (ER beta)
• Masse moléculaire calculée: 59 kDa
• Numéro d’acquisition GenBank: BC024181
• Symbole du gène: ESR2
• Identification du gène (NCBI): 2100
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.

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