Anticorps Polyclonal de lapin anti-ESR2 Beta 6

ESR2 Beta 6 Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF,ELISA

Conjugaison

Non conjugué

N° de cat : 55471-1-AP

Synonymes

ER BETA, Erb, ESR BETA, ESR2, ESR2 Beta 6, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, NR3A2

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Galerie de données de validation

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  WB analysis of mouse spleen using 55471-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 55471-1-AP (ESR2 Beta-6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 55471-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 55471-1-AP (ESR2 Beta-6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 55471-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 55471-1-AP (ESR2 Beta-6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 55471-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55471-1-AP (ESR2 Beta-6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 55471-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55471-1-AP (ESR2 Beta-6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 55471-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55471-1-AP (ESR2 Beta-6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervix using 55471-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 55471-1-AP (ESR2 Beta-6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervix using 55471-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 55471-1-AP (ESR2 Beta-6 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 55471-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ESR2 Beta 6 antibody (55471-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red), DAPI (Blue).

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Applications testées

• Résultats positifs en WB: tissu splénique de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

55471-1-AP cible ESR2 Beta 6 dans les applications de WB, IHC, IF,ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: estrogen receptor 2 (ER beta)
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: NM_001437
• Symbole du gène: ESR2
• Identification du gène (NCBI): 2100
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual