Anticorps Monoclonal anti-ESRRB

ESRRB Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3E10C1

N° de cat : 66818-1-PBS

Synonymes

3E10C1, ERR beta-2, ERRb, ERRB2, ERRbeta

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Galerie de données de validation

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  WB analysis using 66818-1-Ig (same clone as 66818-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66818-1-Ig (ESRRB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66818-1-PBS in a different storage buffer formulation.

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  WB analysis using 66818-1-Ig (same clone as 66818-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66818-1-Ig (ESRRB antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66818-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66818-1-Ig (same clone as 66818-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66818-1-Ig (ESRRB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66818-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66818-1-Ig (same clone as 66818-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66818-1-Ig (ESRRB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66818-1-PBS in a different storage buffer formulation.

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  IF Staining of Caco-2 using 66818-1-Ig (same clone as 66818-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using ESRRB antibody (66818-1-Ig, Clone: 3E10C1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), Alpha Tubulin antibody (80762-1-RR, Clone: 1G7, red). This data was developed using the same antibody clone with 66818-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Caco-2 using 66818-1-Ig (same clone as 66818-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using ESRRB antibody (66818-1-Ig, Clone: 3E10C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66818-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66818-1-PBS cible ESRRB dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ESRRB Protéine recombinante Ag18626
• Nom complet: estrogen-related receptor beta
• Masse moléculaire calculée: 508 aa, 56 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC131517
• Symbole du gène: ESRRB
• Identification du gène (NCBI): 2103
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Estrogen related receptor beta (ESRRB), a NR3 Steroid Receptor, has been shown to affect early placental development. Also it can act as a repressor of transcriptional activity mediated by the glucocorticoid receptor (GR). It binds as a monomer to the extended half-site TNAAGTGCA and as a homodimer to the estrogen response element (ERE), palindromic thyroid hormone response element (TRE(pal)), and SF-1 response element, but not to the glucocorticoid response element (GRE). Estrogen related receptor beta mutations lead to abnormal development of the chorion and defective diploid trophoblast proliferation, and are lethal during midgestation. Expression has been documented in mice in the extra embryonic ectoderm that gives rise to the chorion. ESTs have been isolated from normal human eye, lung, and testis libraries. The ligand for the receptor is PPARgamma coactivator 1 (PGC-1) beta. ESRRB exists various isoforms and molecular weight of each isoform is 48 kDa,55 kDa and 56 kDa.

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