Anticorps Monoclonal anti-ESYT2

• Phare
• Validé par KD/KO

ESYT2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B12G2

N° de cat : 68374-1-PBS

Synonymes

FAM62B, Chr2Syt, E Syt2, E-Syt2, Extended synaptotagmin 2

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Galerie de données de validation

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  WB analysis using 68374-1-Ig (same clone as 68374-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68374-1-Ig (FAM62B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68374-1-PBS in a different storage buffer formulation.

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  WB analysis using 68374-1-Ig (same clone as 68374-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68374-1-Ig (ESYT2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68374-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68374-1-Ig (same clone as 68374-1-PBS)

  WB result of FAM62B antibody (68374-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAM62B transfected HeLa cells. This data was developed using the same antibody clone with 68374-1-PBS in a different storage buffer formulation.

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  WB analysis using 68374-1-Ig (same clone as 68374-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 68374-1-Ig (FAM62B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68374-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 68374-1-Ig (same clone as 68374-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ESYT2 antibody (68374-1-Ig, Clone: 1B12G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68374-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68374-1-PBS cible ESYT2 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ESYT2 Protéine recombinante Ag20440
• Nom complet: family with sequence similarity 62 (C2 domain containing) member B
• Masse moléculaire calculée: 921 aa, 102 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: BC013957
• Symbole du gène: ESYT2
• Identification du gène (NCBI): 57488
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ESYT2, also named FAM62B, is one of the three-member family of Extended Synaptotagmins that were recently shown to be implicated in the formation of endoplasmic reticulum (ER)-plasma membrane (PM) junctions and in the Ca(2+) dependent regulation of these junctions.

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