Anticorps Monoclonal anti-ETFA

ETFA Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3C12G5

N° de cat : 67661-1-PBS

Synonymes

3C12G5, Alpha ETF, Alpha-ETF, EMA, GA2

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Galerie de données de validation

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  WB analysis of mouse brain using 67661-1-Ig (same clone as 67661-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67661-1-Ig (ETFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67661-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67661-1-Ig (same clone as 67661-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67661-1-Ig (ETFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67661-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67661-1-Ig (same clone as 67661-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67661-1-Ig (ETFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67661-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67661-1-Ig (same clone as 67661-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67661-1-Ig (ETFA antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67661-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67661-1-Ig (same clone as 67661-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67661-1-Ig (ETFA antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67661-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67661-1-Ig (same clone as 67661-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ETFA antibody (67661-1-Ig, Clone: 3C12G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). DAPI (blue). This data was developed using the same antibody clone with 67661-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67661-1-PBS cible ETFA dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ETFA Protéine recombinante Ag30257
• Nom complet: electron-transfer-flavoprotein, alpha polypeptide
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC015526
• Symbole du gène: ETFA
• Identification du gène (NCBI): 2108
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The electron transfer flavoprotein(ETF), located in the mitochondrial matrix, is a nuclear-encoded enzyme delivering to the respiratory chain by straight-chain acyl-CoA dehydrogenases and other dehydrogenases. Mitochondrial fatty acids oxidation and amino acids metabolism are affected in MADD(Multiple acyl-CoA dehydrogenase deficiency). These disorders are linked to a dysfunction of ETF. ETF is a mitochondrial matrix protein consisting of alpha- (30kDa) and beta- (28kDa) subunits encoded by the ETFA and ETFB genes, respectively. (PMID: 31996215,PMID: 16510302)

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