Anticorps Polyclonal de lapin anti-ETS1

• Phare
• Validé par KD/KO

ETS1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12118-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 12118-1-AP (same clone as 12118-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12118-1-AP (ETS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 12118-1-AP (same clone as 12118-1-PBS)

  WB result of ETS1 antibody (12118-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ETS1 transfected Jurkat cells. This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 12118-1-AP (same clone as 12118-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12118-1-AP (ETS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 12118-1-AP (same clone as 12118-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12118-1-AP (ETS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

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  IHC staining of rat spleen using 12118-1-AP (same clone as 12118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 12118-1-AP (ETS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

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  IHC staining of rat spleen using 12118-1-AP (same clone as 12118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 12118-1-AP (ETS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

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  IHC staining of rat lung using 12118-1-AP (same clone as 12118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 12118-1-AP (ETS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat lung using 12118-1-AP (same clone as 12118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 12118-1-AP (ETS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 12118-1-AP (same clone as 12118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 12118-1-AP (ETS1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 12118-1-AP (same clone as 12118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 12118-1-AP (ETS1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 12118-1-AP (same clone as 12118-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ETS1 antibody (12118-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12118-1-PBS cible ETS1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ETS1 Protéine recombinante Ag2127
• Nom complet: v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)
• Masse moléculaire calculée: 441 aa, 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC017314
• Symbole du gène: ETS1
• Identification du gène (NCBI): 2113
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ETS1 is a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. ETS1 is a nuclear protein and primarily acts as a transcriptional activator, though it can also repress gene transcription. Since it is primarily expressed in lymphocytes, it plays several critical roles in immunity. In addition, it is important for angiogenesis. Since cells express several Ets proteins at the same time, functional redundancy is possible. This is particularly shown for the socalled housekeeping genes where several Ets factors were found to occupy the proximal promoters in vivo. The ETS1 protein exists as three isoforms: ETS1-p51, ETS1-p42, ETS1-p27 ranging in size from 27 to 51 kDa.

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