Anticorps Monoclonal anti-ETS1

• Phare
• Validé par KD/KO

ETS1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IHC, IF/ICC, ChIP, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

4F2D4

N° de cat : 66598-1-Ig

Synonymes

Protein C-ets-1, EWSR2, ETS 1, 4F2D4

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Galerie de données de validation

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  WB analysis of HepG2 using 66598-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66598-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 66598-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66598-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat spleen using 66598-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 66598-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 66598-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 66598-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 66598-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 66598-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 66598-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 66598-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 66598-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 66598-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 66598-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ETS1 antibody (66598-1-Ig, Clone: 4F2D4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Daudi, cellules HSCT6, cellules Jurkat
• Résultats positifs en IHC: tissu splénique de rat, tissu d'intestin grêle de rat, tissu d'intestin grêle de souris, tissu pulmonaire de rat, tissu pulmonaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:5000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

66598-1-Ig cible ETS1 dans les applications de WB, IHC, IF/ICC, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ETS1 Protéine recombinante Ag2127
• Nom complet: v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)
• Masse moléculaire calculée: 441 aa, 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC017314
• Symbole du gène: ETS1
• Identification du gène (NCBI): 2113
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ETS1 is a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. ETS1 is a nuclear protein and primarily acts as a transcriptional activator, though it can also repress gene transcription. Since it is primarily expressed in lymphocytes, it plays several critical roles in immunity. In addition, it is important for angiogenesis. Since cells express several Ets proteins at the same time, functional redundancy is possible. This is particularly shown for the socalled housekeeping genes where several Ets factors were found to occupy the proximal promoters in vivo.

Publications

Species	Application	Title
human	WB	Cell Death Dis; PLA2G7 promotes immune evasion of bladder cancer through the JAK-STAT-PDL1 axis; Authors - Ding Peng
human	WB,IHC	Mol Med; ets1 associates with KMT5A to participate in high glucose-mediated EndMT via upregulation of PFN2 expression in diabetic nephropathy.; Authors - Lihong Lu ; KD Validated
human	WB	Blood Adv; ETS1 is a novel transcriptional regulator of adult T-cell leukemia/lymphoma of North American descent; Authors - Rebecca A Luchtel
human	WB	Nat Microbiol; FEAR antiviral response pathway is independent of interferons and countered by poxvirus proteins; Authors - Emily A Rex
human	WB,IHC,ChIP	Int J Biol Sci; ETS1-mediated Regulation of SOAT1 Enhances the Malignant Phenotype of Oral Squamous Cell Carcinoma and Induces Tumor-associated Macrophages M2-like Polarization; Authors - Yueying Liu
human,mouse	WB	Elife; Neurons enhance blood-brain barrier function via upregulating claudin-5 and VE-cadherin expression due to glial cell line-derived neurotrophic factor secretion; Authors - Lu Yang ; KD Validated

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