Anticorps Monoclonal anti-ETS1

• Phare
• Validé par KD/KO

ETS1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4F2D4

N° de cat : 66598-1-PBS

Synonymes

Protein C-ets-1, EWSR2, ETS 1, 4F2D4

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Galerie de données de validation

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  WB analysis of HepG2 using 66598-1-Ig (same clone as 66598-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66598-1-Ig (same clone as 66598-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Daudi using 66598-1-Ig (same clone as 66598-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 66598-1-Ig (same clone as 66598-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 66598-1-Ig (same clone as 66598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 66598-1-Ig (same clone as 66598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 66598-1-Ig (same clone as 66598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 66598-1-Ig (same clone as 66598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 66598-1-Ig (same clone as 66598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 66598-1-Ig (same clone as 66598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat lung using 66598-1-Ig (same clone as 66598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat lung using 66598-1-Ig (same clone as 66598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat small intestine using 66598-1-Ig (same clone as 66598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat small intestine using 66598-1-Ig (same clone as 66598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66598-1-Ig (ETS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 66598-1-Ig (same clone as 66598-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ETS1 antibody (66598-1-Ig, Clone: 4F2D4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66598-1-PBS cible ETS1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ETS1 Protéine recombinante Ag2127
• Nom complet: v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)
• Masse moléculaire calculée: 441 aa, 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC017314
• Symbole du gène: ETS1
• Identification du gène (NCBI): 2113
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ETS1 is a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. ETS1 is a nuclear protein and primarily acts as a transcriptional activator, though it can also repress gene transcription. Since it is primarily expressed in lymphocytes, it plays several critical roles in immunity. In addition, it is important for angiogenesis. Since cells express several Ets proteins at the same time, functional redundancy is possible. This is particularly shown for the socalled housekeeping genes where several Ets factors were found to occupy the proximal promoters in vivo.

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