Anticorps Recombinant de lapin anti-ETV4

ETV4 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241042E10

N° de cat : 83891-6-PBS

Synonymes

PEA3, ETS translocation variant 4, E1A-F, E1AF, Adenovirus E1A enhancer-binding protein

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Galerie de données de validation

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  WB analysis of K-562 using 83891-6-RR (same clone as 83891-6-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 83891-6-RR (ETV4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83891-6-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 83891-6-RR (same clone as 83891-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83891-6-RR (ETV4 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83891-6-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 83891-6-RR (same clone as 83891-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83891-6-RR (ETV4 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83891-6-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83891-6-RR (same clone as 83891-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ETV4 antibody (83891-6-RR, Clone: 241042E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83891-6-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83891-6-RR (same clone as 83891-6-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug ETV4 Recombinant antibody (83891-6-RR, Clone:241042E10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83891-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83891-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83891-6-RR against Human ETV4 were performed. The affinity constant is 0.157 nM.

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Informations sur le produit

83891-6-PBS cible ETV4 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ETV4 Protéine recombinante Ag0984
• Nom complet: ets variant 4
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 61-65 kDa
• Numéro d’acquisition GenBank: BC007242
• Symbole du gène: ETV4
• Identification du gène (NCBI): 2118
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ETS translocation variant 4 (ETV4), also named as E1A-F or PEA3, is 484 amino acid protein, which contains one ETS DNA-binding domain and belongs to the ETS family. The calculated molecular weight of ETV4 protein is 54 kDa, but the phosphorylated ETV4 is a 54-61 kDa protein. ETV4 as a transcription activator localizes in the nucleus and binds to the enhancer of the adenovirus E1A gene.

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