Anticorps Polyclonal de lapin anti-ETV5

• Phare
• Validé par KD/KO

ETV5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13011-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 13011-1-AP (same clone as 13011-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13011-1-AP (ETV5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13011-1-PBS in a different storage buffer formulation.

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  WB analysis using 13011-1-AP (same clone as 13011-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13011-1-AP (ETV5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13011-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13011-1-AP (same clone as 13011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13011-1-AP (ETV5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13011-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13011-1-AP (same clone as 13011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13011-1-AP (ETV5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13011-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 13011-1-AP (same clone as 13011-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 13011-1-AP (ETV5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13011-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13011-1-PBS cible ETV5 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ETV5 Protéine recombinante Ag3708
• Nom complet: ets variant 5
• Masse moléculaire calculée: 510 aa, 58 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC007333
• Symbole du gène: ETV5
• Identification du gène (NCBI): 2119
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ETS variant gene 5(ETV5) belongs to the ETS oncogene family that shares a conserved peptide, ETS domain, which mediates sequence-specific DNA binding. ETV5 is a transcription factor and is required for spermatogonial stem cell self-renewal. ETV5 can be negatively regulated by COP1, a tumor suppressor. Also it has a role in branching morphogenesis in the developing kidney

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