Anticorps Polyclonal de lapin anti-EWS

EWS Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 55191-1-PBS

Synonymes

EWSR1, EWS oncogene, RNA binding protein EWS, RNA-binding protein EWS

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Galerie de données de validation

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  WB analysis using 55191-1-AP (same clone as 55191-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55191-1-AP (EWS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 55191-1-AP (same clone as 55191-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 55191-1-AP (EWS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 55191-1-AP (same clone as 55191-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55191-1-AP (EWS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 55191-1-AP (same clone as 55191-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55191-1-AP (EWS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 55191-1-AP (same clone as 55191-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 55191-1-AP (EWS Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 55191-1-AP (same clone as 55191-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 55191-1-AP (EWS Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 55191-1-AP (same clone as 55191-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55191-1-AP (EWS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

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Informations sur le produit

55191-1-PBS cible EWS dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Ewing sarcoma breakpoint region 1
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 68-70 kDa
• Numéro d’acquisition GenBank: NM_005243
• Symbole du gène: EWS
• Identification du gène (NCBI): 2130
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EWS, also named as WSR1, belongs to the RRM TET family. It might normally function as a repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. This antibody is specific to EWS. And a 90 kDa band also could be detected (PMID:23552423).

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