Anticorps Recombinant de lapin anti-EXO1/Exonuclease 1

EXO1/Exonuclease 1 Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251210D3

N° de cat : 86445-1-PBS

Synonymes

EXO1, EC:3.1.-.-, EXOI, Exonuclease 1, Exonuclease I

---

Galerie de données de validation

• 

  WB analysis using 86445-1-RR (same clone as 86445-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86445-1-RR (EXO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86445-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA sample test of MP02742-1

  The mean EXO1/Exonuclease 1 concentration was determined to be 4.80 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load.

• 

  Sandwich ELISA standard curve of MP02742-1

  Sandwich ELISA standard curve of MP02742-1, Human EXO1/Exonuclease 1 Recombinant Matched Antibody Pair - PBS only. 86445-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag9253. 86445-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

• 

  IF Staining of HEK-293 using 86445-1-RR (same clone as 86445-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using EXO1 antibody (86445-1-RR, Clone: 251210D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86445-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

86445-1-PBS cible EXO1/Exonuclease 1 dans les applications de WB, IF/ICC, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: EXO1/Exonuclease 1 Protéine recombinante Ag9253
• Nom complet: exonuclease 1
• Masse moléculaire calculée: 94 kDa
• Poids moléculaire observé: 115-120 kDa
• Numéro d’acquisition GenBank: BC007491
• Symbole du gène: EXO1
• Identification du gène (NCBI): 9156
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

{{ptg:RelatedPrimaryAntibodies}}