Anticorps Polyclonal de lapin anti-EXOC5

• Phare
• Validé par KD/KO

EXOC5 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 17593-1-AP

Synonymes

EXOC5, exocyst complex component 5, HSEC10, PRO1912, SEC10, SEC10L1, SEC10P

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Galerie de données de validation

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  WB analysis of mouse brain using 17593-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17593-1-AP (EXOC5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human adrenal gland using 17593-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 17593-1-AP (EXOC5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17593-1-AP

  IP Result of anti-EXOC5 (IP:17593-1-AP, 3ug; Detection:17593-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human testis using 17593-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17593-1-AP (EXOC5 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 17593-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17593-1-AP (EXOC5 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 17593-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17593-1-AP (EXOC5 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 17593-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17593-1-AP (EXOC5 antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 17593-1-AP

  Immunofluorescent analysis of HepG2 cells, using EXOC5 antibody 17593-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu de glande surrénale humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

17593-1-AP cible EXOC5 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EXOC5 Protéine recombinante Ag11524
• Nom complet: exocyst complex component 5
• Masse moléculaire calculée: 708 aa, 82 kDa
• Poids moléculaire observé: 77 kDa
• Numéro d’acquisition GenBank: BC041126
• Symbole du gène: EXOC5
• Identification du gène (NCBI): 10640
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EXOC5 (SEC10), a component of the exocyst complex, is a 77-kDa protein with a broad tissue distribution (PubMed: 9119050). The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. The complex is also essential for the biogenesis of epithelial cell surface polarity.

Publications

Species	Application	Title
human	WB	Cell; RalB and the exocyst mediate the cellular starvation response by direct activation of autophagosome assembly.; Authors - Bodemann Brian O BO ; KD Validated
mouse	WB	Elife; Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis; Authors - Kunli Zhao
human	WB	Sci Rep; Insights into the ubiquitin-proteasome system of human embryonic stem cells.; Authors - Isabel Saez
human	WB,IHC	Mol Cancer Res; EXOC4 Promotes Diffuse-Type Gastric Cancer Metastasis via Activating FAK Signal.; Authors - Haojie Li ; KD Validated
mouse	IF	Cells; The Exocyst Is Required for CD36 Fatty Acid Translocase Trafficking and Free Fatty Acid Uptake in Skeletal Muscle Cells; Authors - Nicole K. Nakamura
mouse	WB	J Biol Chem; Disrupted glucose homeostasis and skeletal muscle-specific glucose uptake in an exocyst knockout mouse model.; Authors - Brent A Fujimoto ; KO Validated

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