Anticorps Polyclonal de lapin anti-EXOSC10

• Phare
• Validé par KD/KO

EXOSC10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(8)

Avis (2)

N° de cat : 11178-1-AP

Synonymes

Rrp6p, RRP6, PM/Scl-100, P100 polymyositis-scleroderma overlap syndrome-associated autoantigen, Exosome complex component 10

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Galerie de données de validation

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  WB analysis of HeLa using 11178-1-AP

  WB result of EXOSC10 antibody (11178-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC10 transfected HeLa cells.

• 

  IP experiment of MCF-7 using 11178-1-AP

  IP result of anti-EXOSC10 (IP:11178-1-AP, 4ug; Detection:11178-1-AP 1:300) with MCF-7 cells lysate 3200 ug.

• 

  IHC staining of human breast cancer using 11178-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11178-1-AP (EXOSC10 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 11178-1-AP

  Immunofluorescent analysis of NIH/3T3 cells using 11178-1-AP (EXOSC10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa,
• Résultats positifs en IP: cellules MCF-7,
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

11178-1-AP cible EXOSC10 dans les applications de WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EXOSC10 Protéine recombinante Ag1666
• Nom complet: exosome component 10
• Masse moléculaire calculée: 98 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC039901
• Symbole du gène: EXOSC10
• Identification du gène (NCBI): 5394
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a neuclear
ucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl 2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl paitents. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.

Publications

Species	Application	Title
human	IP	Nat Commun; TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.; Authors - Roy Matkovic
human	WB	Cell Rep; Human Telomerase RNA Processing and Quality Control.; Authors - Chi-Kang Tseng ; KD Validated
mouse	WB,RIP	Development; Post-transcription regulation by the exosome complex is required for cell survival and forebrain development by repressing P53 signaling.; Authors - Pauline Antonie Ulmke ; KO Validated
human	IF	RNA; Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.; Authors - Alan L Jiao
human	WB	Life Sci Alliance; Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication; Authors - Tobias Moll
human	WB	Cancer Res; NAT10 drives cisplatin chemoresistance by enhancing ac4C-associated DNA repair in bladder cancer; Authors - Ruihui Xie

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Roy (Verified Customer) (06-12-2024)

Did detect EXOSC10 by WB (1/1000 - overnight incubation at 4°C) in HeLa cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa

FH

Tsimafei (Verified Customer) (12-17-2023)

Antibody detect Exosc10 (around 100 kDa on the image). However non-specific bands are observed in higher molecular weight area

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mESCs

EXOSC10 Antibody Western Blot validation (1:2000 dilution) in mESCs (Cat no:11178-1-AP)

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