Anticorps Polyclonal de lapin anti-EXOSC10

• Phare
• Validé par KD/KO

EXOSC10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11178-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 11178-1-AP (same clone as 11178-1-PBS)

  WB result of EXOSC10 antibody (11178-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC10 transfected HeLa cells. This data was developed using the same antibody clone with 11178-1-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 11178-1-AP (same clone as 11178-1-PBS)

  IP result of anti-EXOSC10 (IP:11178-1-AP, 4ug; Detection:11178-1-AP 1:300) with MCF-7 cells lysate 3200 ug. This data was developed using the same antibody clone with 11178-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 11178-1-AP (same clone as 11178-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11178-1-AP (EXOSC10 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11178-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 11178-1-AP (same clone as 11178-1-PBS)

  Immunofluorescent analysis of NIH/3T3 cells using 11178-1-AP (EXOSC10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11178-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11178-1-PBS cible EXOSC10 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EXOSC10 Protéine recombinante Ag1666
• Nom complet: exosome component 10
• Masse moléculaire calculée: 98 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC039901
• Symbole du gène: EXOSC10
• Identification du gène (NCBI): 5394
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a neuclear
ucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl 2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl paitents. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.

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