Anticorps Monoclonal anti-EXOSC10

EXOSC10 Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

1D6G9

N° de cat : 68315-1-PBS

Synonymes

1D6G9, EC:3.1.13.-, Exosome complex component 10, P100 polymyositis-scleroderma overlap syndrome-associated autoantigen, PM/Scl-100

---

Galerie de données de validation

• 

  WB analysis using 68315-1-Ig (same clone as 68315-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68315-1-Ig (EXOSC10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68315-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 68315-1-Ig (same clone as 68315-1-PBS)

  IP result of anti-EXOSC10 (IP:68315-1-Ig, 4ug; Detection:68315-1-Ig 1:1000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 68315-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 68315-1-Ig (same clone as 68315-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EXOSC10 antibody (68315-1-Ig, Clone: 1D6G9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68315-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

68315-1-PBS cible EXOSC10 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EXOSC10 Protéine recombinante Ag28587
• Nom complet: exosome component 10
• Masse moléculaire calculée: 98 kDa
• Poids moléculaire observé: 98 kDa
• Numéro d’acquisition GenBank: BC039901
• Symbole du gène: EXOSC10
• Identification du gène (NCBI): 5394
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a neuclear/nucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl 2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl paitents. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.

{{ptg:RelatedPrimaryAntibodies}}