Anticorps Polyclonal de lapin anti-EXOSC2

• Phare
• Validé par KD/KO

EXOSC2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (2)

N° de cat : 14805-1-AP

Synonymes

EXOSC2, exosome component 2, hRrp4p, p7, RRP4, Rrp4p

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Galerie de données de validation

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  WB analysis of HEK-293 using 14805-1-AP

  WB result of EXOSC2 antibody (14805-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC2 transfected HEK-293 cells.

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  WB analysis of HeLa using 14805-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14805-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14805-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14805-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14805-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14805-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14805-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14805-1-AP

  IP Result of anti-EXOSC2 (IP:14805-1-AP, 4ug; Detection:14805-1-AP 1:1000) with HeLa cells lysate 1080ug.

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  IHC staining of human skin cancer using 14805-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 14805-1-AP (EXOSC2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin cancer using 14805-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 14805-1-AP (EXOSC2 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of MCF-7 using 14805-1-AP

  Immunofluorescent analysis of MCF-7 cells, using EXOSC2 antibody 14805-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules MCF-7
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer de la peau humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

14805-1-AP cible EXOSC2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EXOSC2 Protéine recombinante Ag6525
• Nom complet: exosome component 2
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 30-33 kDa
• Numéro d’acquisition GenBank: BC000747
• Symbole du gène: EXOSC2
• Identification du gène (NCBI): 23404
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snoRNA and snRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs [PMID:15346807]. EXOSC2 is a non-catalytic component of the RNA exosome complex that has 3'->5' exoribonuclease activity and involves in a multitude of cellular RNA processing and degradation events [PMID: 17545563].

Publications

Species	Application	Title
human	WB,IF	Cell; Modulated Expression of Specific tRNAs Drives Gene Expression and Cancer Progression.; Authors - Hani Goodarzi
human		Cell; The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest.; Authors - Jakob Trendel
human	WB	Life Sci Alliance; Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication; Authors - Tobias Moll
human	WB	Mol Biotechnol; EXOSC2 Mediates the Pro-tumor Role of WTAP in Breast Cancer Cells via Activating the Wnt/β-Catenin Signal; Authors - Chen-Guang Lv ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tsimafei (Verified Customer) (12-04-2022)

50µg of total protein were loaded on a gel. Incubation ON at 4C

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: mESCs

EXOSC2 Antibody Western Blot validation (1:5000 dilution) in mESCs (Cat no:14805-1-AP)

FH

Nikolaus (Verified Customer) (07-14-2022)

Antibodies were incubated ON at 4C.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mESCs

EXOSC2 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:14805-1-AP)