Anticorps Polyclonal de lapin anti-EXOSC4

• Phare
• Validé par KD/KO

EXOSC4 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 15937-1-AP

Synonymes

EXOSC4, exosome component 4, hRrp41p, p12A, RRP41, RRP41A, Rrp41p, SKI6, Ski6p

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Galerie de données de validation

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  WB analysis using 15937-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15937-1-AP (EXOSC4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15937-1-AP

  WB result of EXOSC4 antibody (15937-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC4 transfected Jurkat cells.

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  IHC staining of human skin cancer using 15937-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15937-1-AP (EXOSC4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 15937-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15937-1-AP (EXOSC4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 15937-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15937-1-AP (EXOSC4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 15937-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15937-1-AP (EXOSC4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15937-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EXOSC4 antibody (15937-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 15937-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EXOSC4 antibody (15937-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HCT 116, cellules HeLa, cellules Jurkat, cellules SW480, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer de la peau humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

15937-1-AP cible EXOSC4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EXOSC4 Protéine recombinante Ag7595
• Nom complet: exosome component 4
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC002777
• Symbole du gène: EXOSC4
• Identification du gène (NCBI): 54512
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EXOSC4, also named as RRP41 and SKI6, belongs to the RNase PH family. EXOSC4 is a novel component of the human exosome complex. EXOSC4 is the most important RNA processing machinery in maturation and degradation of RNA playing a major role in gene expression regulation (PMID: 29626590). EXOSC4 has 1 isoform with the molecular mass of 27 kDa. However, studies have shown that EXOSC4 can interact with EXOSC2 and EXOSC7, possibly form a complex with the molecular weight of 90 kDa (PMID: 25115889). Recently, EXOSC4 has been found to play important roles in tumorigcncsis and development including CRC (PMID: 33075373).

Publications

Species	Application	Title
human	WB	J Biol Chem; A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement.; Authors - Tzu-Wei Chuang
human	WB,IHC	Life Sci; STX2 drives colorectal cancer proliferation via upregulation of EXOSC4.; Authors - Yong-Xia Wang ; KD Validated
human	WB	Life Sci Alliance; Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication; Authors - Tobias Moll

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tsimafei (Verified Customer) (07-06-2022)

Control and Exosc4 depletion shows a reduction of Exosc4 signal. Antibodies were incubated ON at 4C.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mESCs

EXOSC4 Antibody Western Blot validation (1:2000 dilution) in mESCs (Cat no:15937-1-AP)