Anticorps Recombinant de lapin anti-EXOSC6

EXOSC6 Recombinant Antibody for IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241535G9

N° de cat : 84346-2-PBS

Synonymes

MTR3, hMtr3p, hMtr3, exosome component 6, EAP4

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Galerie de données de validation

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  IF Staining of HeLa using 84346-2-RR (same clone as 84346-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EXOSC6 antibody (84346-2-RR, Clone: 241535G9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84346-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01222-1

  Cytometric bead array standard curve of MP01222-1, EXOSC6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84346-2-PBS. Detection antibody: 84346-1-PBS. Standard: Ag33513. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84346-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84346-2-RR against Human EXOSC6 were performed. The affinity constant is 0.399 nM.

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Informations sur le produit

84346-2-PBS cible EXOSC6 dans les applications de IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: EXOSC6 Protéine recombinante Ag33513
• Nom complet: exosome component 6
• Masse moléculaire calculée: 28kd
• Poids moléculaire observé: 28-32 kDa
• Numéro d’acquisition GenBank: NM_058219
• Symbole du gène: EXOSC6
• Identification du gène (NCBI): 118460
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EXOSC6 (exosome component 6), also known as p11 or MTR3. The calculated molecular weight of EXOSC6 is 28 kDa. And it has low tissue specificity. The gene product constitutes one of the subunits of the multisubunit particle called exosome, which mediates mRNA degradation. It is also a component of the RNA exosome complex (PMID: 29906447). The composition of human exosome is similar to its yeast counterpart. EXOSC6 is homologous to the yeast Mtr3 protein. Its exact function is not known, however, it has been shown using a cell-free RNA decay system that the exosome is required for rapid degradation of unstable mRNAs containing AU-rich elements (AREs), but not for poly(A) shortening. The exosome does not recognize ARE-containing mRNAs on its own, but requires ARE-binding proteins that could interact with the exosome and recruit it to unstable mRNAs, thereby promoting their rapid degradation.

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