Anticorps Polyclonal de lapin anti-EXOSC8

• Phare
• Validé par KD/KO

EXOSC8 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (2)

N° de cat : 11979-1-AP

Synonymes

CIP3, EAP2, EXOSC8, exosome component 8, OIP 2, OIP2, Opa interacting protein 2, p9, RP11 421P11.3, RRP43, Rrp43p

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Galerie de données de validation

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  WB analysis of HEK-293 using 11979-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11979-1-AP (EXOSC8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11979-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11979-1-AP (EXOSC8 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11979-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11979-1-AP (EXOSC8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11979-1-AP

  IP Result of anti-EXOSC8 (IP:11979-1-AP, 4ug; Detection:11979-1-AP 1:500) with HEK-293 cells lysate 1560ug.

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  IHC staining of human testis using 11979-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11979-1-AP (EXOSC8 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 11979-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11979-1-AP (EXOSC8 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 11979-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11979-1-AP (EXOSC8 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 11979-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11979-1-AP (EXOSC8 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 11979-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11979-1-AP (EXOSC8 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 11979-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11979-1-AP (EXOSC8 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of 4T1 using 11979-1-AP

  Immunofluorescent analysis of 4T1 cells, using EXOSC8 antibody 11979-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, tissu cérébral humain
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu testiculaire humain, tissu de cancer du pancréas humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules 4T1

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

11979-1-AP cible EXOSC8 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EXOSC8 Protéine recombinante Ag2605
• Nom complet: exosome component 8
• Masse moléculaire calculée: 276 aa, 30 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: BC020773
• Symbole du gène: EXOSC8
• Identification du gène (NCBI): 11340
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EXOSC8 (exosome component 8), also known as p9 or CIP3, is a component of the exosome multi-enzyme ribonuclease complex, that degrades or processes messenger RNA. It belongs to the RNase PH family and localizes to the nucleolus. EXOSC8 is one of the six RNase-PH domain subunits of the exosome. EXOSC8 is part of the central hexamer channel of the exosome. EXOSC8 mutations could alter mRNA metabolism and cause hypomyelination with spinal muscular atrophy and cerebellar hypoplasia.

Publications

Species	Application	Title
human	WB	Nat Commun; Loss of heterozygosity of essential genes represents a widespread class of potential cancer vulnerabilities.; Authors - Caitlin A Nichols ; KO Validated
human	WB	Nat Commun; EXOSC8 mutations alter mRNA metabolism and cause hypomyelination with spinal muscular atrophy and cerebellar hypoplasia.; Authors - Veronika Boczonadi ; KD Validated
human	WB	PLoS One; Exosome-Bound WD Repeat Protein Monad Inhibits Breast Cancer Cell Invasion by Degrading Amphiregulin mRNA.; Authors - Saeki Makio M
human	WB	Neuromuscul Disord; New subtype of PCH1C caused by novel EXOSC8 variants in a 16-year-old Spanish patient.; Authors - María Elena Rodríguez-García
human	WB	Life Sci Alliance; Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication; Authors - Tobias Moll
human	WB	Nature; Nucleolar URB1 ensures 3' ETS rRNA removal to prevent exosome surveillance; Authors - Lin Shan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tsimafei (Verified Customer) (12-04-2022)

50µg of total protein were used. Incubation ON at 4C

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mESCs

EXOSC8 Antibody Western Blot validation (1:2000 dilution) in mESCs (Cat no:11979-1-AP)

FH

Nikolaus (Verified Customer) (05-24-2022)

Antibodies applied after 2 rounds of stripping, signal still satisfactory

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: mESC

EXOSC8 Antibody Western Blot validation (1/2000 dilution) in mESC (Cat no:11979-1-AP)