Anticorps Polyclonal de lapin anti-EXOSC9

• Phare
• Validé par KD/KO

EXOSC9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (2)

N° de cat : 24470-1-AP

Synonymes

PMSCL1, PM/Scl-75, P75 polymyositis-scleroderma overlap syndrome-associated autoantigen, Exosome complex component RRP45

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Galerie de données de validation

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  WB analysis using 24470-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24470-1-AP (EXOSC9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24470-1-AP

  WB result of EXOSC9 antibody (24470-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC9 transfected HeLa cells.

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  WB analysis of MCF-7 using 24470-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24470-1-AP (EXOSC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate hyperplasia using 24470-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 24470-1-AP (EXOSC9 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse kidney using 24470-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24470-1-AP (EXOSC9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 24470-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EXOSC9 antibody (24470-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IHC: tissu d'hyperplasie de la prostate humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

24470-1-AP cible EXOSC9 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, levure
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EXOSC9 Protéine recombinante Ag19768
• Nom complet: exosome component 9
• Masse moléculaire calculée: 456 aa, 51 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC142978
• Symbole du gène: EXOSC9
• Identification du gène (NCBI): 5393
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EXOSC9, also named as PMSCL1 and PM/Scl-75, is a key subunit of the exosome complex. EXOSC9 maintains self-renewal in a cell-autonomous manner. EXOSC9 has 4 isoforms with MW 49 kDa, 51 kDa, 39 kDa and 41 kDa. It's polymyositis/scleroderma auto-antigen 75 kDa. The MW of EXOSC9 is migrated to 60-75 kDa for modification in WB detection.

Publications

Species	Application	Title
human	WB	Life Sci Alliance; Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication; Authors - Tobias Moll
yeast	WB	G3 (Bethesda); Humanized Saccharomyces cerevisiae provides a facile and effective tool to identify damaging human variants that cause exosomopathies; Authors - Khondakar Sayef Ahammed
human	IF	Mol Cell; Degradation of circular RNA by the ribonuclease DIS3; Authors - Xiao Tao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tsimafei (Verified Customer) (12-17-2023)

Great antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mESCs

EXOSC9 Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:24470-1-AP)

FH

Nikolaus (Verified Customer) (07-14-2022)

Antibodies were incubated ON at 4C. Exosc9 depletion was used as a control to check the specificity of the band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mESCs

EXOSC9 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:24470-1-AP)

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