Anticorps Monoclonal anti-EXOSC9

• Phare
• Validé par KD/KO

EXOSC9 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

3F4G1

Avis (1)

N° de cat : 67636-1-Ig

Synonymes

Autoantigen PM/Scl 1, EXOSC9, exosome component 9, p5, p6, PM/Scl 75, PMSCL1, RRP45, Rrp45p

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Galerie de données de validation

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  WB analysis using 67636-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67636-1-Ig (EXOSC9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HeLa using 67636-1-Ig

  WB result of EXOSC9 antibody (67636-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC9 transfected HeLa cells.

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  IHC staining of human breast cancer using 67636-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67636-1-Ig (EXOSC9 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 67636-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67636-1-Ig (EXOSC9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 67636-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67636-1-Ig (EXOSC9 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67636-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67636-1-Ig (EXOSC9 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67636-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EXOSC9 antibody (67636-1-Ig, Clone: 3F4G1 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules A549, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu rénal de rat, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:750-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67636-1-Ig cible EXOSC9 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EXOSC9 Protéine recombinante Ag19783
• Nom complet: exosome component 9
• Masse moléculaire calculée: 456 aa, 51 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC142978
• Symbole du gène: EXOSC9
• Identification du gène (NCBI): 5393
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EXOSC9, also named as PMSCL1 and PM/Scl-75, is a key subunit of the exosome complex. EXOSC9 maintains self-renewal in a cell-autonomous manner. EXOSC9 has 4 isoforms with MW 49 kDa, 51 kDa, 39 kDa and 41 kDa. It's polymyositis/scleroderma auto-antigen 75 kDa. The MW of EXOSC9 is migrated to 60-75 kDa for modification in WB detection.

Publications

Species	Application	Title
human	WB	Cell Rep; Cytoplasmic DIS3 is an exosome-independent endoribonuclease with catalytic activity toward circular RNAs; Authors - Claudia Latini

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nikolaus (Verified Customer) (07-14-2022)

Antibodies were incubated ON at 4C. Exosc9 depletion was used as a control to check the specificity of the band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mESCs

EXOSC9 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:67636-1-Ig)

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