Anticorps Polyclonal de lapin anti-EXT2

EXT2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11348-1-PBS

Synonymes

EC:2.4.1.224, Exostosin 2, EXT-2, Glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase, Heparan sulfate co-polymerase subunit EXT1

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Galerie de données de validation

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  WB analysis using 11348-1-AP (same clone as 11348-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11348-1-AP (EXT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11348-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 11348-1-AP (same clone as 11348-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11348-1-AP (EXT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11348-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11348-1-AP (same clone as 11348-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11348-1-AP (EXT2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11348-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 11348-1-AP (same clone as 11348-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using EXT2 antibody (11348-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11348-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11348-1-PBS cible EXT2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EXT2 Protéine recombinante Ag1748
• Nom complet: exostoses (multiple) 2
• Masse moléculaire calculée: 718 aa, 82 kDa
• Poids moléculaire observé: 70-82 kDa
• Numéro d’acquisition GenBank: BC010058
• Symbole du gène: EXT2
• Identification du gène (NCBI): 2132
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Exostosin-2 (EXT2) is type II transmembrane glycosyltransferase forming with EXT1 the heterodimeric heparan sulfate polymerase which catalyzes the elongation of the heparan sulfate glycan backbone(PMID: 22660413, 36402845, 36593275).

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