Anticorps Polyclonal de lapin anti-EXTL2

EXTL2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12828-1-PBS

Synonymes

Alpha GalNAcT EXTL2, Alpha-1,4-N-acetylhexosaminyltransferase EXTL2, Alpha-GalNAcT EXTL2, EC:2.4.1.223, exostoses (multiple) like 2

---

Galerie de données de validation

• 

  WB analysis using 12828-1-AP (same clone as 12828-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12828-1-AP (EXTL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12828-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12828-1-AP (same clone as 12828-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12828-1-AP (EXTL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12828-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12828-1-AP (same clone as 12828-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12828-1-AP (EXTL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12828-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 12828-1-AP (same clone as 12828-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12828-1-AP (EXTL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12828-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 12828-1-AP (same clone as 12828-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12828-1-AP (EXTL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12828-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 12828-1-AP (same clone as 12828-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using EXTL2 antibody (12828-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12828-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 12828-1-AP (same clone as 12828-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EXTL2 antibody (12828-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12828-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

12828-1-PBS cible EXTL2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EXTL2 Protéine recombinante Ag3548
• Nom complet: exostoses (multiple)-like 2
• Masse moléculaire calculée: 330 aa, 37 kDa
• Poids moléculaire observé: 37-43 kDa
• Numéro d’acquisition GenBank: BC036015
• Symbole du gène: EXTL2
• Identification du gène (NCBI): 2135
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Exostosin-like 2 (EXTL2) is one of three EXT-like genes in the human genome that are homologous to EXT1 and EXT2, encodes a transferase that adds not only GlcNAc but also N-acetylgalactosamine to the glycosaminoglycan (GAG)-protein linkage region via an α1,4-linkage. Loss of the enzyme EXTL2, a regulatory mechanism serving to limit CSPG synthesis, resulted in more extensive versican deposition into a lysolecithin-induced demyelinated injury, a larger area of myelin disruption with an associated decrease in axonal density within the lesion, and greater accumulation of macrophages and microglia (PMID: 32703234, 23395820)

{{ptg:RelatedPrimaryAntibodies}}